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Written Paper

Genetic variability of isolates of Citrus tristeza virus discovered in Italy in the last 20 years  [2004]

Davino, S. (Catania Univ. (Italy). Dipartimento di Scienze e Tecnologie Fitosanitarie) Guardo, M. (Consiglio per la Ricerca e la Sperimentazione in Agricoltura (CRA) - Istituto Sperimentale per l'Agrumicoltura, Acireale, Catania (Italy)) Caruso, A. (Consiglio per la Ricerca e la Sperimentazione in Agricoltura (CRA) - Istituto Sperimentale per l'Agrumicoltura, Acireale, Catania (Italy)) Davino, M. (Catania Univ. (Italy). Dipartimento di Scienze e Tecnologie Fitosanitarie)

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Since 1982, thousand of citrus plants affected by Citrus tristeza virus (CTV), illegally introduced by foreign Countries, have been detected in Italy. The plants belong to the following species: Alemow, Shamouti, Navelina and Valencia sweet orange, calamondin, Rangpur lime, Ceylon lemon, Fortune and Nova mandarin, grapefruit, satsuma, Minneola tangelo and recently also thousand of local Tarocco. CTV isolates differ for their biological characteristics, particularly for intensity of symptoms on different cultivars, in transmissibility by aphid vectors and in their ability in cross-protection, therefore they are suitable for the study of genetic characteristics of Italian CTV isolates. Genetic variability of CTV isolates have been analized using SSCP, bi-directional PCR and sequencing. The results clearly show that different isolates are referable to mild isolates present since long time in different Countries of the Mediterranean basin, while others are more virulent and more similar to Asians and Americans isolates

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Atti delle Giornate Fitopatologiche (Italy)