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Journal Article

Journal Article

Isolation of a Bacillus licheniformis DK42 Producing Cellulase and Xylanase, and Properties of the Enzymes  [2008]

Kim, M.J. (Dankook University, Cheonan, Republic of Korea); Lim, S.J. (Dankook University, Cheonan, Republic of Korea); Kang, D.K. (Dankook University, Cheonan, Republic of Korea), E-mail: dkkang@dankook.ac.kr;

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A bacterium producing cold-active cellulase and xylanase was isolated from pig feces. The isolate, DK42 strain, was found to be the Gram-positive, non-motile, catalase-positive, and spore-forming stain. Under an electron microscope, the cells were observed to be rod-shaped. The isolate was identified as Bacillus licheniformis DK42 on the basis of morphological and biochemical properties as well as 16S rRNA gene sequences. The characterization of crude cellulase and xylanase from B. licheniformis DK42 was investigated. Cellulase exhibited an optimum temperature and pH at 45℃ and 6.0, whereas xylanase exhibited an optimum temperature and pH at 55℃ and 6.0. Especially cellulase maintained approx. 50% of its maximum activity even at 10℃, indicating that it is cold-active. Both cellulase and xylanase were stable after 2hr at 35℃, whereas they lost their activities after 30min at 65℃.
From the journal
Journal of Animal Science and Technology
ISSN : 1598-9429

Bibliographic information

Language:
Korean
Type:
Directory
In AGRIS since:
2009
Volume:
50
Issue:
3
Start Page:
429
End Page:
436
All titles:
"Isolation of a Bacillus licheniformis DK42 Producing Cellulase and Xylanase, and Properties of the Enzymes"@eng
"Cellulase 및 Xylanase를 분비하는 Bacillus licheniformis DK42의 분리 및 효소 특성"@kor
Other:
"Summary(En)"
"1 tables"
"6ill., 18 ref."
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Bibliographic information

Language:
Korean
Type:
Directory
In AGRIS since:
2009
Volume:
50
Issue:
3
Start Page:
429
End Page:
436
All titles:
"Isolation of a Bacillus licheniformis DK42 Producing Cellulase and Xylanase, and Properties of the Enzymes"@eng
"Cellulase 및 Xylanase를 분비하는 Bacillus licheniformis DK42의 분리 및 효소 특성"@kor
Other:
"Summary(En)"
"1 tables"
"6ill., 18 ref."