Written Paper

Inhibitory Effects of Naringenin and Its Novel Derivatives on Hyaluronidase  [2009]

Moon, S.H., Konkuk University, Seoul, Republic of Korea Kim, K.T., Konkuk University, Seoul, Republic of Korea Lee, N.K., Konkuk University, Seoul, Republic of Korea Han, Y.S., Konkuk University, Seoul, Republic of Korea et al.

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Naringenin is a bioactive flavanone containing antioxidative, anti-inflammatory, and anticarcinogenic properties. The inhibitory effects oil hyaluronidase of naringenin and its novel derivatives were evaluated. Among these flavonoids at 200 μM concentration, 7-O-butyl naringenin had the highest inhibitory effect oil hyaluronidase with 44.84%. In addition, For naringenin at concentrations of 0, 150, and 190 μM, the apparent Michaelis constants (∧appK∧m) were calculated to be 0.60±0.02, 0.43±0.02, and 0.41±0.01 mg/mL of substrate, respectively; for 7-O-butyl naringenin at 0, 20, and 30 μM concentrations, those were 0.44±0.03 and 0.27±0.03 mg/mL, respectively. The V∧max values at 150 and 190 μM naringenin were 0.59±0.02 and 0.56±0.01 mg/mL/min, respectively; and those at 20 and 30 μM 7-O-butyl naringenin were 0.50±0.02 and 0.33±0.02 mg/mL/min, respectively. However, the slopes of each inhibitory reaction were not significantly different. Therefore, naringenin and 7-O-butyl naringenin were shown to be uncompetitive inhibitors. These results demonstrate the potential use of 7-O-butyl naringenin as all anti-inflammatory substance.

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Food Science and Biotechnology

ISSN : 1226-7708