Journal Article
Propolis Protects Menadione-induced Apoptosis in 3T3-L1 Adipocytes by Inhibiting ROS Generation [2012]
Pak, E.J., Kyonggi University, Suwon, Republic of Korea; Moon, J.Y., Kyonggi University, Suwon, Republic of Korea; Yoo, B.S., Kyonggi University, Suwon, Republic of Korea; Yoon, J., Dongnam Health University, Suwon, Republic of Korea;
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Menadione is a commonly used compound that causes oxidative stress in cell. The study was conducted to investigate the effect of propolis on menadione-induced apoptosis of 33-L1 adipocytes. Menadione (40μM) induced morphological change of 3T3-L1 adipocytes, observed under phase contrast microscopy. However, cell morphology of 3T3-L1 adipocytes pretreated with propolis (3㎍/ml was similar to control group, suggesting that propolis can protect against morphological change induced by menadione treatment (40μM) for 6 h. The protective effect of propolis on menadione-induced apoptosis of 3T3-L1 adipocytes was conformed by Hoechst 33258 staining. To investigate the role of ROS in menadione-induced apoptosis and protection by propolis, the generation of ROS was determined in cells by using DCFH-DA staining. The results showed that ROS generation was significantly reduced in cells pretreated with propolis (0.3 and 3㎍/ml). Consequently, propolis protected 3T3-L1 adipocytes against menadione-induced apoptosis, which might be related to the reduction of ROS generation by menadione treatment.
