Journal Article
JQ1, a BET inhibitor, controls TLR4-induced IL-10 production in regulatory B cells by BRD4-NF-kB axis
[2017]
Lee, M.B., Konkuk University, Chungju, Republic of Korea;
Lee, J.H., Konkuk University, Chungju, Republic of Korea;
Hong, S.H., Konkuk University, Chungju, Republic of Korea;
You, J.S., Konkuk University, Chungju, Republic of Korea;
et al.
JQ1, a BET inhibitor, controls TLR4-induced IL-10 production in regulatory B cells by BRD4-NF-kB axis
2017
Lee, M.B., Konkuk University, Chungju; Lee, J.H., Konkuk University, Chungju; Hong, S.H., Konkuk University, Chungju; You, J.S., Konkuk University, Chungju; Nam, S.T., Konkuk University, Chungju; Kim, H.W., Konkuk University, Chungju; Park, Y.H., Konkuk University, Chungju; Lee, D., Konkuk University, Chungju; Min, K.Y., Konkuk University, Chungju; Park, Y.M., Konkuk University, Chungju; Kim, Y.M., Duksung Women's University, Seoul; Kim, H.S., Konkuk University, Chungju; Choi, W.S., Konkuk University, Chungju
Regulatory B cells, also well-known as IL-10-producing B cells, play a role in the suppression of inflammatory responses. However, the epigenetic modulation of regulatory B cells is largely unknown. Recent studies showed that the bromodomain and extra-terminal domain (BET) protein inhibitor JQ1 controls the expression of various genes involving cell proliferation and cell cycle. However, the role of BET proteins on development of regulatory B cells is not reported. In this study, JQ1 potently suppressed IL-10 expression and secretion in murine splenic and peritoneal B cells. While bromodomain-containing protein 4 (BRD4) was associated with NF-κB on IL-10 promoter region by LPS stimulation, JQ1 interfered the interaction of BRD4 with NF-κB on IL-10 promoter. In summary, BRD4 is essential for toll like receptor 4 (TLR4)-mediated IL-10 expression, suggesting JQ1 could be a potential candidate in regulating IL-10-producing regulatory B cells in cancer.
[Biochemistry and Molecular Biology reports]
2018/KR/KR2018_0.rdf
Regulatory B cells, also well-known as IL-10-producing B cells, play a role in the suppression of inflammatory responses. However, the epigenetic modulation of regulatory B cells is largely unknown. Recent studies showed that the bromodomain and extra-terminal domain (BET) protein inhibitor JQ1 controls the expression of various genes involving cell proliferation and cell cycle. However, the role of BET proteins on development of regulatory B cells is not reported. In this study, JQ1 potently suppressed IL-10 expression and secretion in murine splenic and peritoneal B cells. While bromodomain-containing protein 4 (BRD4) was associated with NF-κB on IL-10 promoter region by LPS stimulation, JQ1 interfered the interaction of BRD4 with NF-κB on IL-10 promoter. In summary, BRD4 is essential for toll like receptor 4 (TLR4)-mediated IL-10 expression, suggesting JQ1 could be a potential candidate in regulating IL-10-producing regulatory B cells in cancer.
