The aim of the present study was advanced to evaluate the dynamics of immunoglobulin A, G, M and lactoferrin concentration and somatic cell count in cow milk in relation with the cow seasonal keeping and pathogens presence in the udder. Objectives of the study: (1) to evaluate the functional status (health) of the cow udder by estimating somatic cell count and presence of pathogenic bacteria in milk during the housing and grazing period of the herd, (2) to estimate the change of immunoglobulin A, G and M concentration in the cow milk and blood serum in relation with the cows’ seasonal keeping and presence of pathogenic bacteria in the udder, (3) to analyze the lactoferrin concentration in the udder quarters milk in cows with and without pathogenic agents causing mastitis during the housing and grazing period, (4) to find out the possible correlation between the immunoglobulin A, G, M, lactoferrin concentration, and somatic cell count in milk.