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It is the biggest library of agricultural literature in Latvia, the deposit library of the UN Food and Agriculture Organisation and the national centre of the information systems AGRIS and CARIS.

Journal Article

Journal Article

Retrotransposon-like sequences expressed under various stressors in Pine genome shows different similarity level to known mobile genetic elements  [2012]

Voronova-Petrova, A., Latvian State Forest Research Inst. Silava, Salaspils (Latvia). Genetic Resource Centre; Jansons, A., Latvian State Forest Research Inst. Silava, Salaspils (Latvia). Div. of Forest Tree Breeding; Rungis, D., Latvian State Forest Research Inst. Silava, Salaspils (Latvia). Genetic Resource Centre; .;

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Retrotransposon activity could be a major factor in genome instability, rearrangements and therefore also plasticity of the plant genome and adaptation to changing environmental conditions. This study examines retrotransposon-like sequences in the expression profile of stressed Scots pine (Pinus sylvestris L.) trees. Our initial results suggest the existence of several groups of active retrotransposons in the Scots pine genome, which share differing levels of similarity with known elements from other plant species (Voronova et al., 2011). Naturally occurring stresses such as heat shock and insect infection as well as treatment with specific chemicals like abscisic acid and salicylic acid were tested. Sequence analyses and mobile element identification was performed by searching within several nucleotide and protein databases. Retrotransposon-like sequences are widely transcribed as many are also present in EST (Expressed Sequence Tag) databases of various plant species. Searches of nucleotide sequence databases reveal several elements that have characteristic structural features of full-length LTR (Long Terminal Repeat) retrotransposons. The expressed retrotransposon-like sequences isolated after exposure to different stresses were oft en found to belong to different families of mobile elements. Specific primers were used to evaluate expression of retrotransposonlike sequences between samples subjected to various stresses. The amplification with specific p
rimers shows cross amplification of some fragments. It has not been clarified, how many copies from the identified retrotransposon-like sequences are activated in the complex pine genome, as translation of even a single element can cause transcription of many more nonautonomous retrotransposons with truncated sequences. Further studies of retrotransposon activation in pine could increase understanding of genomic rearrangements in response to stress conditions.

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Bibliographic information

Language:
English
Type:
Conference
In AGRIS since:
2013
Volume:
25
Issue:
58
Start Page:
175
All titles:
" Retrotransposon-like sequences expressed under various stressors in Pine genome shows different similarity level to known mobile genetic elements "
Other:
" Summary only "

From the journal

Speciālais izdevums

ISSN : 1407-270X