Written Paper

Comparative in vivo study of the antifungal effect against Candida albicans and wound healing property of durian (Durio zibethinus Murr.) seed extract versus Nystatin

Abdul, E.A. Abiera, E.M. Alvarez, A.T. Cueco, M.L. et al.

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This in vivo study compares the antifungal and wound healing effects of Durio zibethinus Murr. seeds extract versus Nystatin on full skin thickness wounds. Three full skin thickness incised wounds were inflicted on the lateral surface of each of twelve rabbits. Candida albicans was inoculated on all three wounds of individual rabbits. Wounds were categorized into treatment groups: Nystatin was used for treatment Group A (positive control), Distilled water for Treatment B (negative control) and 100% Durian seeds extract for Treatment C. Daily wound care involved application of the assigned treatment for the wounds followed by sterile gauze dressing. Wound contraction was measured each day over a seven-day observation period. On the third day, wounds were examined grossly using Inflammatory Phase Grading System. On the seventh day, wounds were grossly examined using Fibroplasia Phase Grading System. The inflammatory phase grading system utilized on the third post wounding day indicated better antifungal and wound healing effect for Nystatin on the basis of redness, necrotic tissues and exudates compared to that durian seeds extract. On the seventh post-wounding day, all wounds under Nystatin were entirely dry, and therefore belong to same Grade using Fibroplasia Phase Grading System. However, wounds under durian seeds extract still has unhealed wounds. Based on the results of this study, there was significant difference in the antifungal and wound healing pr
operty of Nystatin and durian seeds extract. Although durian seeds extract exhibited an antifungal effect on C. albicans, however the antifungal effect of Nystatin proved to be more superior than that of the extract