Bibliography
Regulation of gene expression in rice (Oryza sativa L.) infected with rice tungro bacilliform virus [2010]
Balidion, J.F., Philippines Univ. Los Baños, College, Laguna (Philippines);
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Rice tungro disease (RTD) is the most damaging viral disease of rice in South and Southeast Asia. Severe symptoms are expressed when two viruses namely; Rice tungro bacilliform virus (RTBV) and Rice tungro spherical virus (RTSV) infect the plant. Rice plants singly infected with RTBV usually show mild yellow to orange discoloration of the leaf tips and stunting. In order to characterize host responses of Taichung Native 1 (TN1), and the backcross line TW16 (BC5F8), resistant to RTBV infection developed by serial backcrosses of the resistant donor cv. Utri Merah (accession number 16682, UM82) to the recurrent parent TN1 at the gene expression level, time course transcriptome changes in RTBV-infected rice plants were examined using a customized 60-mer oligonucleotide microarray system. Changes in gene expression of RTBV-infected plants revealed that the genes for DNA replication, protein synthesizing machinery and energy production in the mitochondrion were activated by RTBV infection while the genes for photosynthesis, chloroplast synthesis and the synthesis of cell wall structure were suppressed in the resistant and susceptible genotypes. The degrees of induction or repression, however, were higher in the susceptible TN1 than in the resistant TW16. Though transcription activity of genes related to host defense systems under hormone signals and gene silencing were activated in both genotypes at a relatively early stage of infection, these seemed to be insuf
ficient to reduce virus accumulation in TN1 in contrast to that of TW16. Observations on the temporal changes in virus concentration, symptom development and transcriptome changes in RTBV infected plants suggest that regulation of gene expression by the host were largely determined by virus accumulation and symptom development through time.
