Cotton is highly susceptible to soil compaction. Subsoiling effectively alleviates compaction and recovers soil productivity. A study was conducted to evaluate the effect of subsoiling on soil physical properties as bulk density and penetration resistance as well as cotton yield. Three soil tillage treatments viz. S0: moldboard plow (conventional tillage), S1: 30-35 cm subsoiling followed by moldboard plow, and S2: 50- 55 cm subsoiling followed by moldboard plow were randomized in complete block design with four replicates. Results indicated that subsoiling created significant effect on percentage of cone penetration decrease within depths of 10-20 and 20-50 cm (P less than 0.01). Highest average plant height was associated with 50-55 cm subsoiling treatments. Subsoiling resulted in yield increase within 9.7-13.5% and led to improvement of the soil physical properties.