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Written Paper

Diagnosis of avain paramyxovirus type-1 infection in chicken by immunoflourescence technique  [2011]

Shahzad, M. (University of Agriculture, Faisalabad (Pakistan). Dept. of Pathology) Rizvi, F. (University of Agriculture, Faisalabad (Pakistan). Dept. of Pathology) Khan, A. (University of Agriculture, Faisalabad (Pakistan). Dept. of Pathology) Siddique, M. (University of Agriculture, Faisalabad (Pakistan). Dept. of Microbiology) et al.

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Immunoflourescent and histopathological studies, gross lesions and clinical signs were observed in experimentally Newcastle disease virus (NDV) infected broiler chicks. From 4th day post inoculation (DPI), chicks were anorexic, suffered from greenish diarrhea showed respiratory signs with nasal discharge and difficult breathing. Torticulus and in-coordination was also seen during this period. Tracheal hemorrhages and splenomegaly was seen after 4th DPI. Alveoli of lungs were filled with edematous fluid and infiltrated with mononuclear cells. Lymphocytic degeneration in bursa and lymphoid hyperplasia in spleen were recorded on 8th and 10th DPI. Hyper immune serum against NDV was raised in rabbits, purified and globulins were conjugated with fluorescence iso-thiocyanate for immunoflourescent staining of slides for the detection of virus. Slides were deparaffinized, stained with FITC-conjugated antibodies to see under fluorescent microscope. Immu nofluorescence was seen in trachea, lungs, spleen and bursa from 4th DPI at various rats characterized as low, moderate and high.

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International Journal of Agriculture and Biology (Pakistan)

ISSN : 1560-8530

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