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National Agricultural Research Centre (NARC), Islamabad  established in 1984, is the largest research centre of the Pakistan Agricultural Research Council (PARC). NARC, with a total land area of approximately 1400 acres, is located near Rawal Lake, six kilometers south-east of Islamabad. Physical facilities in term of experimental fields, laboratories, green houses, gene bank, library/ documentation, auditorium, machinery & lab equipment repair workshops, stores, hostels, cafeteria, audio visual studios, are also available at NARC.

Journal Article

Journal Article

Evaluation of BSK-H complete medium supplemented with rabbit serum and sodium bicarbonate for the growth of Borrelia anserina  [2013]

Aslam, B. (University of Agriculture, Faisalabad (Pakistan). Inst. of Microbiology); Hussain, I. (University of Agriculture, Faisalabad (Pakistan). Inst. of Microbiology); Mahmood, M.S. (University of Agriculture, Faisalabad (Pakistan). Inst. of Microbiology); Khan, A. (University of Agriculture, Faisalabad (Pakistan). Dept. of Pathology);

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The present study was conducted to compare the effect of 3 formulations Barbour-Stoenner-Kelly (BSK) medium on the growth of Borrelia (B.) anserina, the causative agent of avian borreliosis. Three different formulations of BSK medium (BSK-H, BSK-II and BSK-H complete medium) were prepared. For the isolation of B. anserina, Argas ticks were inoculated in all the three (BSK-H, BSK-II and BSKH complete) formulations of BSK medium. All the samples were also observed for the impact of BSK medium on the growth (Generation time, Growth per hour, Specific growth rate) of B. anserina. Phase contrast microscopy was performed for the observation of viable B. anserina cells, and additional confirmation of all the isolates was done by performing indirect immunofluorescence assay and PCR. BSK-H complete medium supplemented with 6% rabbit serum and sodium bicarbonate was found best when compared to two other formulations of BSK medium with respect to the isolation, generation time and growth rate of Borrelia spirochetes.
From the journal
Pakistan Veterinary Journal (Pakistan)
ISSN : 0253-8318

Bibliographic information

Type:
Journal Article
In AGRIS since:
2014
Volume:
33
Issue:
2
Start Page:
183
End Page:
186
All titles:
"Evaluation of BSK-H complete medium supplemented with rabbit serum and sodium bicarbonate for the growth of Borrelia anserina"@eng
Other:
"3 ills., 1 table, 17 ref., Summary (En), E-mail: driftikharuaf@gmail.com"
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Bibliographic information

Type:
Journal Article
In AGRIS since:
2014
Volume:
33
Issue:
2
Start Page:
183
End Page:
186
All titles:
"Evaluation of BSK-H complete medium supplemented with rabbit serum and sodium bicarbonate for the growth of Borrelia anserina"@eng
Other:
"3 ills., 1 table, 17 ref., Summary (En), E-mail: driftikharuaf@gmail.com"