Written Paper
Antioxidant activity, insect repellent properties and synthesis of chalcones and aurones: cyclization of chalcones into aurones by crude onion and potato enzyme extracts [2009]
Majdalany, M.; Centre International de Hautes Etudes Agronomiques Mediterraneennes,Chania (Greece). Institut Agronomique Mediterraneen [Corporate Author]
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The aim of this study was to synthesize aurones from the corresponding chalcones using the peroxidase and polyphenol oxidase enzymes extracted from onion and potato solid waste in a way to reduce the cost of using the pure enzymes, and evaluate the antioxidant, antifeedant and lipoxygenase inhibitory activity of the synthesized chalcones and aurones. The peroxidase was extracted from the apical trimmings of an onion bulb using buffer pH 4, whereas the polyphenol oxidase was extracted from the peels of a potato tuber using buffer pH 6.6. The tested chalcones have been synthesized using the Claisen- Schmid condensation reaction between appropriately substituted 2-hydroxy-acetophenones and benzaldehydes. To evaluate their antioxidant activity, the TCPO chemiluminescene method was used and the IC50 for each compound were calculated. In order to obtain a better insight of the antioxidant activity of the synthesized chalcones and aurones other tests were also performed: Interaction with the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) stable free radical, measurement of superoxide radical (O2-.) scavenging activity, evaluation of the HO. radical scavenging activity and inhibition of the lipid peroxidation induced by the thermal free radical producer 2,2�-azobis(2amidinopropane) dihydrochloride (AAPH) . Moreover, the ability of the compounds to inhibit the soybean lipoxygenase enzyme was evaluated. Concerning the antifeedant activity, the experiment was performed ag
ainst the pine scale Marchalina hellenica Gennadius (Hemiptera, Sternorhyncha, Margarodidae), the bark beetle Orthotomicus erosus Wollaston (Coleoptera, Scolytidae) and the red palm weevil Rhynchophorus ferrugineus Olivier (Coleoptera, Curculionidae).The insecticidal activity was tested on the number of eggs laid by the females and on the percentage of mortality of the larvae after hatching.
