Written Paper

Evaluation of antiviral activity of fractionated extracts of sage Salvia officinalis L. (Lamiaceae)  [2008]

Smidling, D., Torlak Institute of Immunology and Virusology, Belgrade (Serbia) Mitic-Culafic, D. Vukovic-Gacic, B. Simic, D. et al.

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In the present study, we examined cytotoxicity and extracellular and intracellular antiviral activity of fractionated extracts of wild and cultivated sage Salvia officinalis L. (Lamiaceae) in vitro using the WISH-VSV (human amnion epithelial cells - vesicular stomatitis virus) model system. Extracts were obtained by fractionating depigmented ethanol extracts of sage plants with supercritical CO2 at different pressures. Cytotoxicity was determined by examining cellular morphology in situ with the aid of a colorimetric micromethod and by cell staining with trypan blue. The fraction of distilled cultivated sage obtained at CO2 pressure of 300 bars and temperature of 60 deg C (149/3) was the most cytotoxic, with CTD10 (10% cytotoxic concentration) 44 microgram/ml. That of non-distilled cultivated sage obtained at CO2 pressure of 500 bars and temperature of 100 deg C (144/5) was the least toxic (CTD10 199 microgram/ml). Moreover, 144/5 had an antiviral effect at the intracellular level: when added 5 hours before VSV (vesicular stomatitis virus) infection, it caused 100% reduction of CPE (cytopathic effect) at concentrations of 99.5 and 199.0 microgram/ml; when added after virus penetration had occurred, the same concentrations caused 35 and 60% reduction, respectively. The obtained results indicated that antiviral activity of 144/5 involves inhibition of the early steps of the virus infective cycle without a direct virucidal effect.

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Archives of Biological Sciences (Serbia)

ISSN : 0354-4664