Journal Article
Inheritance of mid-oleic acid trait in sunflower hybrid seeds of the second and third generation [2018]
Demurin, Ya.N.; Borisenko, О.М.; Chebanova, Yu.V., The V.S. Pustovoyt All-Russia Research and Development Inst. of Oil–Producing Crops, Krasnodar (Russian Federation);
Access the full text
We studied regularities of inheritance of the mid oleic content in of F2 and F3 seeds for scientific provision of sunflower breeding for oil quality improvement. Five constant inbred lines of the sunflower genetic collection of All-Russian Research Institute of Oil Crops (Krasnodar) with different oleic acid contents were used: low oleic RHA416 and LG28 (33%), increased oleic VK678 (48%), mid oleic LG27 (66%) and high oleic LG26 (88%, Ol mutation). We determined fatty acid composition by gas-liquid chromatography of methyl esters on a Chrom-5 chromatograph. When crossing mid oleic LG27 with low oleic RHA416 there was not differences between reciprocal F2 in the type of inheritance of oleic acid content in individual seeds. This indicates the absence of a maternal inheritance of the trait of mid oleic content. Negative transgression is established. The single-peak distribution of F2 values, as well as those coinciding with the mean values, indicates the additive nature of inheritance of the trait. When crossing the mid oleic LG27 with an increased oleic VK678, the reciprocal F2 showed no maternal inheritance of the mid oleic content with an additive effect. In the reciprocal F2 of crosses mid oleic LG27 and high oleic LG26, the same type of segregation was observed for two discrete phenotypic classes, the mid oleic and the high oleic. In both F2, there is overdomination of the trait with a coefficient of h/d = 1.2, which is expressed in a positive transgres
sion with the appearance of seeds in the super-high-oleic phenotypic class from 90 to 95%. This is due to the recombination association in the F2 genes of high oleic and mid oleic content. In crosses of LG27 with low oleic LG28 and normal VK678, the correlation coefficient of oleic acid content rF2-F3 varied from unvalued to a positive value of 0.54. In hybridization with the high oleic line of LG26, the correlation coefficient rF2-F3 took only high positive values to 0.88.
