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Agroinstitut Nitra, state enterprise is almost 50 years deparmently educational institution of the Ministry of Agriculture and Rural Development of the Slovak republic. It provides a broad portfolio of services mainly in the area of continuing vocational lifelong education, training and certification of Agricultural advisors, compiles specialized library collection, ensures effective and efficient access to information and information resources through information services and information technology. Portfolio of services is completed by hotel [...]

Journal Article

Journal Article

Quercetin and its microbial degradation product 3,4-dihydroxyphenylacetic acid generate hydrogen peroxide modulating their stability under in vitro conditions  [2009]

Skrbek, S., University of Karlsruhe, Karlsruhe (Germany). Institute of Applied Biosciences, Section of Food Toxicology; Rüfer, C. E., University of Karlsruhe, Karlsruhe (Germany). Institute of Applied Biosciences, Section of Food Toxicology; Marko, D., University of Karlsruhe, Karlsruhe (Germany). Institute of Applied Biosciences, Section of Food Toxicology; Esselen, M., University of Karlsruhe, Karlsruhe (Germany). Institute of Applied Biosciences, Section of Food Toxicology;

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In contrast to the microbial degradation of quercetin (QUE), little is known about its degradation under cell culture conditions and the contribution of potential degradation products to the observed cellular effects of QUE. In a cell culture medium, the concentration of QUE was found to decrease rapidly in a time-dependent manner. The presence of catalase substantially affected the stability of QUE but not of the microbial degradation product, 3,4-dihydroxyphenyl¬acetic acid (HPA). Within human colon carcinoma cells (HT29), formation of a QUE glucuronide, the only metabolite, was observed with a maximum at 3 h, whereas no free QUE was detected under the chosen experimental conditions. From the putative degradation products, only the microbial QUE-metabolite HPA, which was not generated under cell culture conditions, exhibited substantial growth-inhibitory properties in HT29 cells. Both QUE and HPA led to the formation of hydrogen peroxide in the cell culture medium. The presence of catalase or ascorbic acid was found to diminish the growth-inhibitory effect of HPA in cell culture, but also under these conditions, still substantial growth inhibition was observed. However, HPA and phloroglucinol lacked the effectiveness of QUE against the epidermal growth factor receptor and cAMP-hydrolysing phosphodiesterases, indicating a different pattern of cellular activities.

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Bibliographic information

Language:
English
Type:
Bibliography
In AGRIS since:
2009
Volume:
48
Issue:
3
Start Page:
129
End Page:
140
All titles:
" Quercetin and its microbial degradation product 3,4-dihydroxyphenylacetic acid generate hydrogen peroxide modulating their stability under in vitro conditions "
Other:
" Summary (En) "
" 7ill., 62 ref. "

From the journal

Journal of Food and Nutrition Research (Slovak Republic)

ISSN : 1336-8672