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Agroinstitut Nitra, state enterprise is almost 50 years deparmently educational institution of the Ministry of Agriculture and Rural Development of the Slovak republic. It provides a broad portfolio of services mainly in the area of continuing vocational lifelong education, training and certification of Agricultural advisors, compiles specialized library collection, ensures effective and efficient access to information and information resources through information services and information technology. Portfolio of services is completed by hotel [...]

Journal Article

Journal article

Sensitivity to antibiotics in strains of staphylococcus aureus isolated from cows milk  [2010]

Pukácová, J., Regional Veterinary and Food Administration, Dolný Kubín (Slovak Republic); Polaková, L.; Dudriková, E.;

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Strains of Staphylococcus aureus (S. aureus) were isolated from individual milk samples of 500 lacting cows from different areas in Slovakia (PH 1 – Košice region, 300 samples; PH 2 – Žilina region, 200 samples). The statistical significance between both dairy farms included in the experiment in the presence of genus Staphylococcus isolated on Baird-Parker agar from milk samples was p less than 0,0001. Totally, 122 milk samples were positive for the presence of Staphylococcus aureus (83 Košice regions, 52 Žilina regions). All 122 isolates of S. aureus were sensitive for the methicilin as detected by interpretative criteria developed by NCCLS (2002). For 122 S. aureus isolates, we compared antibiotic susceptibility results determined by the standardized agar diffusion assay with the PCR assay for the detection of antibiotic resistance mecA gene. For all isolates, we found a correlation between the results of the PCR and those of classical resistance testing. The obtained results were confirmed by PCR analysis, according to which, any of our tested isolate of S. aureus from all 122 individual milk samples from both experimental dairy farms were not positive for the presence of mecA gene coding the methicilin resistance.

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Potravinárstvo (Slovak Republic)

ISSN : 1338-0230