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University of the West Indies (Regional centre for Bahamas, Barbados, Belize, British Virgin Islands, Grenada, Guyana, Jamaica, Montserrat, Netherlands Antilles, St. Kitts and Nevis, St. Lucia, St. Vincent and the Grenadines and Trinidad and Tobago)

Journal Article

Journal Article

Mass production of virus-free patchouli plants [Pogostemon cablin (Blanco) Benth.] by in vitro culture  [1990]

Kukreja, A.K.; Mathur, A.K.; Zaim, M. (Central Institute of Medicinal and Aromatic Plants, Lucknow (India));

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A simple, defined medium has been standardized for the mass multiplication and production of virus-free plants of Pogostemon cablin (Blanco) Benth. Shoot meristems cultured on Murashige and Skoog's (1962) medium (MS) supplemented with 2.0 mg 1**-1 6-benzylaminopurine (BAP) or 0.5-2.0 mg 1**-1 6-y,y-dimethylallyaminopurine (2iP) or 0.5-2.0 mg 1**-1 zeatin (Z) and 1.0 mg 1**-1 indole-3-acetin acid (IAA) exhibited multiple shoot proliferation. Regenerated shoots readily rooted on half strength MS containing 1.0 mg 1**-1 IAA or alpha-naphthaleneacetic acid (NAA) and 1.5 % sucrose. Rooted plantlets were transferred to pots, hardened in the glasshouse and grown to maturity in the field with a high rate (80-90 %) of survival. Plant tests made for patchouli mosaic virus (PaMV) with extracts of plants developed in vitro from the fifth subculture passage onwards gave a negative reaction in diagnostic tests on the local lesion host plant, Chenopodium quinoa Willd., demonstrating the absence of the causal agent in plants regenerated from the shoot meristems.
From the journal
Tropical Agriculture (Trinidad and Tobago)
ISSN : 0041-3216

Bibliographic information

Language:
English
Type:
Summary
In AGRIS since:
1991
Volume:
67
Issue:
2
Start Page:
101
End Page:
104
All titles:
"Mass production of virus-free patchouli plants [Pogostemon cablin (Blanco) Benth.] by in vitro culture"@eng
Other:
"1 ill., 21 ref. Summary (En) *XG"
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Bibliographic information

Language:
English
Type:
Summary
In AGRIS since:
1991
Volume:
67
Issue:
2
Start Page:
101
End Page:
104
All titles:
"Mass production of virus-free patchouli plants [Pogostemon cablin (Blanco) Benth.] by in vitro culture"@eng
Other:
"1 ill., 21 ref. Summary (En) *XG"