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The National Agricultural Library is one of four national libraries of the United States, with locations in Beltsville, Maryland and Washington, D.C. It houses one of the world's largest and most accessible agricultural information collections and serves as the nexus for a national network of state land-grant and U.S. Department of Agriculture field libraries. In fiscal year 2011 (Oct 2010 through Sept 2011) NAL delivered more than 100 million direct customer service transactions.

Journal Article

Journal Article

Structure and antitumor activities of the water-soluble polysaccharides from Ganoderma tsugae mycelium  [2005]

Peng, Y.; Zhang, L.; Zeng, F.; Kennedy, J.F.;

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Six water-soluble polysaccharides coded as GTM1 to GTM6 were extracted sequentially from the mycelium of Ganoderma tsugae with 0.2 M sodium phosphate buffer solution at 25, 40, and 80 degrees C, water at 120 degrees C, 0.5 M sodium hydroxide at 25 and 65 degrees C. The chemical structures were determined by using IR, EA, GC and 13C NMR. The weight-average molecular mass (M(w)) was characterized by size exclusion chromatography combined with laser light scattering (SEC-LLS). The results indicated that the samples GTM1 and GTM2 were heteropolysaccharide-protein complexes with the protein content of 13.5 and 20.1%, and apparent-mean M(w) of 62.8 x 10(4), 81.8 x 10(4), respectively. GTM3 and GTM4 contained (1 leads to 3)-beta-D-glucans and (1 leads to 4)-alpha-D-glucans, while GTM5 and GTM6 were mainly a (1 leads to 6)-branched (1 leads to 3)-beta-D-glucan. Two peaks were found in the SEC patterns of the four samples GTM1 to GTM4, representing fractions 1 composed of branched (1 leads to 4)-alpha-D-glucan with high M(w) and fraction 2 composed of galactose, glucose and mannose with relatively low M(w). With the progress of isolation, the content of fraction 2 decreased from 90.2 to 57.1%, accompanying with enhancing antitumor activity. The polysaccharides GTM1, GTM2 and GTM3 had significantly higher antitumor activity against solid tumor Sarcoma 180 with the inhibition ratio beyond 50%. The results suggested that the effects of the moderate content of galactos
e and bound protein, as well as relatively lower M(w), on the improvement of antitumor activities of polysaccharides could not be negligible.

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Bibliographic information

Language:
English
Type:
Journal Article
In AGRIS since:
2013
Volume:
59
Issue:
3
Start Page:
385
End Page:
392
All titles:
" Structure and antitumor activities of the water-soluble polysaccharides from Ganoderma tsugae mycelium "
Other:
" Includes references "

From the journal

Carbohydrate polymers

ISSN : 0144-8617