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The National Agricultural Library is one of four national libraries of the United States, with locations in Beltsville, Maryland and Washington, D.C. It houses one of the world's largest and most accessible agricultural information collections and serves as the nexus for a national network of state land-grant and U.S. Department of Agriculture field libraries. In fiscal year 2011 (Oct 2010 through Sept 2011) NAL delivered more than 100 million direct customer service transactions.

Journal Article

Journal article

Apoptosis of human breast cancer cells induced by hemagglutinin from Phaseolus vulgaris cv. Legumi secchi  [2011]

Lam, Sze Kwan; Ng, Tzi Bun;

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A dimeric hemagglutinin with a relative molecular mass of 62000 was purified from seeds of Phaseolus vulgaris cultivar “Legumi secchi”. The chromatographic protocol included the use of Blue-Sepharose, Q-Sepharose, and Superdex 75. The N-terminal amino acid sequence of the hemagglutinin resembled those of other Phaseolus hemagglutinins. Its hemagglutinating activity was stable from 0°C to 60°C, and pH 4-11. It inhibited HIV-1 reverse transcriptase (IC₅₀ of 17.3μM) and suppressed the proliferation of breast cancer MCF-7 cells (IC₅₀ of 0.2μM). However, it was devoid of antifungal activity. The hemagglutinin-treated MCF-7 cells showed a number of changes, including cell cycle arrest in G2/M phase, phosphatidylserine externalisation and mitochondrial membrane depolarisation. The hemagglutinin-induced apoptosis through the death receptor-mediated pathway involved Fas ligands, caspase-8 activation, BID truncation, p53 release, caspase-9 activation and Lamin A/C truncation. The understanding of the apoptotic mechanism may be of value for application in tumor therapy.

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Food chemistry

ISSN : 0308-8146