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The National Agricultural Library is one of four national libraries of the United States, with locations in Beltsville, Maryland and Washington, D.C. It houses one of the world's largest and most accessible agricultural information collections and serves as the nexus for a national network of state land-grant and U.S. Department of Agriculture field libraries. In fiscal year 2011 (Oct 2010 through Sept 2011) NAL delivered more than 100 million direct customer service transactions.

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Journal Article

Journal Article

Targeted Protease Substrate for a Quantitative Determination of Protease Activities in the Endolysosomal Pathway  [2006]

Fischer, Rainer; Bächle, Daniel; Fotin-Mleczek, Mariola; Jung, Günther; et al.

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Inside the cell, proteases act in concert in the degradation of proteins and peptides. In order to understand the significance of an individual proteolytic activity within an ensemble of proteases, protocols and probes are required that enable a quantitative determination of the contribution of a protease to the break-down of a given substrate. Here we present a fluorescence resonance energy transfer-based probe and protocols for a quantitative determination of proteolytic activities inside the endolysosomal compartment. A peptide substrate that is readily cleaved by different cathepsins is flanked by fluorescein and tetramethylrhodamine-labeled lysine residues. Efficient endolysosomal targeting of the substrate is achieved by N-terminal elongation with the cell-penetrating peptide nona-arginine. The proteasome inhibitor lactacystin has a small, but significant effect on the break-down of the substrate, thus demonstrating that only a minor fraction of the peptide reaches the cytoplasm in its intact form. Nona-arginine therefore constitutes a highly efficient low-molecular-weight moiety for targeting the endolysosomal compartment.
From the journal
Chembiochem
ISSN : 1439-4227

Bibliographic information

Language:
English
Type:
Journal Article
In AGRIS since:
2013
Volume:
7
Issue:
9
Start Page:
1428
End Page:
1434
Publisher:
Wiley-VCH Verlag
All titles:
"Targeted Protease Substrate for a Quantitative Determination of Protease Activities in the Endolysosomal Pathway"@eng
Other:
"Includes references"
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Bibliographic information

Language:
English
Type:
Journal Article
In AGRIS since:
2013
Volume:
7
Issue:
9
Start Page:
1428
End Page:
1434
Publisher:
Wiley-VCH Verlag
All titles:
"Targeted Protease Substrate for a Quantitative Determination of Protease Activities in the Endolysosomal Pathway"@eng
Other:
"Includes references"