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The National Agricultural Library is one of four national libraries of the United States, with locations in Beltsville, Maryland and Washington, D.C. It houses one of the world's largest and most accessible agricultural information collections and serves as the nexus for a national network of state land-grant and U.S. Department of Agriculture field libraries. In fiscal year 2011 (Oct 2010 through Sept 2011) NAL delivered more than 100 million direct customer service transactions.

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Journal Article

Journal Article

Pseudo-embryogenic structures in anther and isolated microspore cultures in vitro: a cautionary guide  [2012]

Bal, Ugur; Shariatpanahi, Mehran E.; Castro, Antonio J.; Emery, Delphine; et al.

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This review describes sources of structures of non-microspore origin observed in anther and microspore cultures. Various characteristics of these structures may cause a wrong diagnosis of these structures as embryos or cell/tissue clusters of microspore origin. Here we suggest such structures to be named as pseudo-embryogenic structures. The introduction of pseudo-embryogenic structures and their origins could be helpful to distinguish them from true microspore-derived structures. Prompted by certain environmental cues, somatic cells existing as a contamination in immature pollen (microspores) cultures can lead to the formation of ‘pseudo-embryos’ commonly known as embryoids. The pseudo-embryogenic structures may be classified in the following groups: (i) pseudo-star-like structures; pseudo-multicellular structures; (ii) pseudo-embryos with pseudo-suspensors; (iii) contaminating bacteria appearing as callus colonies; (iv) calli and embryos of somatic origin; (v) giant tetrad-like structures; (vi) anther wall cells. The exact origin of these structures is discussed in this paper, and some recommendations are proposed in order to avoid misinterpretation.
From the journal
Czech journal of genetics and plant breeding
ISSN : 1212-1975

Bibliographic information

Language:
English
Type:
Journal Article
In AGRIS since:
2013
Volume:
48
Issue:
2
Start Page:
51
End Page:
60
All titles:
"Pseudo-embryogenic structures in anther and isolated microspore cultures in vitro: a cautionary guide"@eng
Other:
"Includes references"
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Bibliographic information

Language:
English
Type:
Journal Article
In AGRIS since:
2013
Volume:
48
Issue:
2
Start Page:
51
End Page:
60
All titles:
"Pseudo-embryogenic structures in anther and isolated microspore cultures in vitro: a cautionary guide"@eng
Other:
"Includes references"