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The National Agricultural Library is one of four national libraries of the United States, with locations in Beltsville, Maryland and Washington, D.C. It houses one of the world's largest and most accessible agricultural information collections and serves as the nexus for a national network of state land-grant and U.S. Department of Agriculture field libraries. In fiscal year 2011 (Oct 2010 through Sept 2011) NAL delivered more than 100 million direct customer service transactions.

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Journal Article

Journal Article

Identification and enumeration of Salmonella on sample slides of poultry carcass wash water using image analysis with fluorescent microscopy  [1999]

Huang, J.; Li, Y.; Slavik, M.F.; Tao, Y.; et al.

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Rapid detection of bacteria on poultry carcasses is desirable for the poultry industry to enhance food safety of poultry products. This research focused on image analysis with fluorescent microscopy for identification and enumeration of Salmonella typhimurium on sample slides of poultry carcass wash water which were prepared using fluorescent antibodies and immuno-magnetic beads. The criteria of morphological and optical characteristics of S. typhimurium cells, including area, aspect ratio, diameter, major and minor axis, maximum and minimum radii perimeter, radius ratio, length and width, and intensity, were developed. An algorithm that included channel extracting, median filtering, image sharpening, image dilation and erosion, image flattening, and watershed filtering was selected using Image-Pro Plus software to analyze the acquired images. The algorithm was trained with 110 slide samples. The regression analysis showed that the relationship between the automatic image counting and the microbial plate counting was linear with a correlation coefficient of 0.88. The detection limit of this method was 1 x 10(4) cells/mL, and the time needed was less than 4 h.
From the journal
Transactions of the ASAE
ISSN : 0001-2351

Bibliographic information

Language:
English
Type:
Journal Article
In AGRIS since:
2013
Volume:
42
Issue:
1
Start Page:
267
End Page:
273
All titles:
"Identification and enumeration of Salmonella on sample slides of poultry carcass wash water using image analysis with fluorescent microscopy"@eng
Other:
"Includes references"
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Bibliographic information

Language:
English
Type:
Journal Article
In AGRIS since:
2013
Volume:
42
Issue:
1
Start Page:
267
End Page:
273
All titles:
"Identification and enumeration of Salmonella on sample slides of poultry carcass wash water using image analysis with fluorescent microscopy"@eng
Other:
"Includes references"