Heartwood samples from Juniperus virginiana, were extracted with liquid carbon dioxide and the bioactivity of carbon dioxide-derived cedarwood oil (CWO) towards several species of arthropods was investigated. Repellency or toxicity was tested for ants, ticks, and cockroaches. Ants in an outdoor bioassay were significantly repelled by the presence of cedarwood oil on a pole leading to a sugar-water solution. Similarly, imported red fire ants were repelled by CWO separating them from a food source. Black legged tick nymphs exhibited dosage dependent mortality when exposed to CWO and at the highest dosage tested the CWO killed 100 percent of the ticks. Adult houseflies exhibited over 90% mortality when exposed to the highest dose of CWO tested in a contact mortality bioassay and there was a clear dosage dependent mortality for houseflies in a topical mortality bioassay. However, the highest amount tested in the topical mortality only reached 60% mortality. The crude CWO extract did show some repellency towards both German and American cockroaches; however, the cedrol by itself appeared somewhat attractive, though the observed result may have been caused by sub-lethal effects causing decreased locomotion out of the treated area. These results together demonstrate a clear potential for the use of CWO as an insect control agent. Although some species were less affected than others, repellency or toxicity for others was extremely promising.