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The National Agricultural Library is one of four national libraries of the United States, with locations in Beltsville, Maryland and Washington, D.C. It houses one of the world's largest and most accessible agricultural information collections and serves as the nexus for a national network of state land-grant and U.S. Department of Agriculture field libraries. In fiscal year 2011 (Oct 2010 through Sept 2011) NAL delivered more than 100 million direct customer service transactions.

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Journal Article

Journal Article

RhoA/ROCK pathway activity is essential for the correct localization of the germ plasm mRNAs in zebrafish embryos  [2016]

Miranda-Rodríguez, Jerónimo Roberto; Enrique Salas-Vidal; Hilda Lomelí; Mario Zurita; et al.

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Zebrafish germ plasm is composed of mRNAs such as vasa and nanos and of proteins such as Bucky ball, all of which localize symmetrically in four aggregates at the distal region of the first two cleavage furrows. The coordination of actin microfilaments, microtubules and kinesin is essential for the correct localization of the germ plasm. Rho-GTPases, through their effectors, coordinate cytoskeletal dynamics. We address the participation of RhoA and its effector ROCK in germ plasm localization during the transition from two- to eight-cell embryos. We found that active RhoA is enriched along the cleavage furrow during the first two division cycles, whereas ROCK localizes at the distal region of the cleavage furrows in a similar pattern as the germ plasm mRNAs. Specific inhibition of RhoA and ROCK affected microtubules organization at the cleavage furrow; these caused the incorrect localization of the germ plasm mRNAs. The incorrect localization of the germ plasm led to a dramatic change in the number of germ cells during the blastula and 24hpf embryo stages without affecting any other developmental processes. We demonstrate that the Rho/ROCK pathway is intimately related to the determination of germ cells in zebrafish embryos.
From the journal
Developmental biology
ISSN : 0012-1606

Bibliographic information

Language:
English
Type:
Journal Article
In AGRIS since:
2017
Volume:
2016,
Start Page:
.
Publisher:
Elsevier Inc.
All titles:
"RhoA/ROCK pathway activity is essential for the correct localization of the germ plasm mRNAs in zebrafish embryos"@eng
Other:
"Pre-press version"
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Bibliographic information

Language:
English
Type:
Journal Article
In AGRIS since:
2017
Volume:
2016,
Start Page:
.
Publisher:
Elsevier Inc.
All titles:
"RhoA/ROCK pathway activity is essential for the correct localization of the germ plasm mRNAs in zebrafish embryos"@eng
Other:
"Pre-press version"