Journal Article
Myricetin attenuates lipopolysaccharideâstimulated activation of mouse bone marrowâderived dendritic cells through suppression of IKK/NFâκB and MAPK signalling pathways [2013]
RuâHuei; Fu; ShihâPing; Liu; et al.
BACKGROUND: Myricetin is a naturally occurring flavonoid that is found in many fruits, vegetables, teas and medicinal herbs. It has been demonstrated to have antiâinflammatory properties, but, to date, no studies have described the immunomodulatory effects of myricetin on the functions of dendritic cells (DCs). The aim of this study was to evaluate the potential for myricetin to modulate lipopolysaccharide (LPS)âstimulated activation of mouse bone marrowâderived DCs. RESULTS: Our experimental data showed that treatment with myricetin up to 10 µg mLâ»Â¹ does not cause cytotoxicity in cells. Myricetin significantly decreased the secretion of tumour necrosis factorâα, interleukinâ6 and interleukinâ12p70 by LPSâstimulated DCs. The expression of LPSâinduced major histocompatibility class II, CD40 and CD86 on DCs was also inhibited by myricetin, and the endocytic and migratory capacity of LPSâstimulated DCs was blocked by myricentin. In addition, LPSâstimulated DCâelicited allogeneic Tâcell proliferation was reduced by myricetin. Moreover, our results confirmed that myricetin attenuates the responses of LPSâstimulated activation of DCs via suppression of IκB kinase/nuclear factorâκB and mitogenâactivated protein kinaseâdependent pathways. CONCLUSION: Myricetin has novel immunopharmacological activity, and modulation of DCs by myricetin may be an attractive strategy for the treatment of inflammatory and autoimmune disorders, a
nd for transplantation. Copyright © 2012 Society of Chemical Industry
