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The National Agricultural Library is one of four national libraries of the United States, with locations in Beltsville, Maryland and Washington, D.C. It houses one of the world's largest and most accessible agricultural information collections and serves as the nexus for a national network of state land-grant and U.S. Department of Agriculture field libraries. In fiscal year 2011 (Oct 2010 through Sept 2011) NAL delivered more than 100 million direct customer service transactions.

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Journal Article

Journal Article

Fermented blueberry juice extract and its specific fractions have an anti-adipogenic effect in 3 T3-L1 cells  [2017]

Sánchez-Villavicencio, Mayra L.; Vinqvist-Tymchuk, Melinda; Kalt, Wilhelmina; Matar, Chantal; et al.

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BACKGROUND: Obesity and Type 2 diabetes have reached epidemic status worldwide. Wild lowbush blueberry (Vaccinium angustifolium Aiton) is a plant of the North American Aboriginal traditional pharmacopeia with antidiabetic potential, especially when it is fermented with Serratia vaccinii. METHODS: A phytochemical fractionation scheme was used to identify potential bioactive compounds as confirmed by HPLC retention times and UV–Vis spectra. 3 T3-L1 cells were differentiated for 7 days with either Normal Blueberry Extract (NBE), Fermented Blueberry Extract (FBE/F1), seven fractions and four pure compounds. Triglyceride content was measured. Examination of selected intracellular signalling components (p-Akt, p-AMPK) and transcriptional factors (SREBP-1c and PPARγ) was carried out by Western blot analysis. RESULTS: The inhibitory effect of FBE/F1 on adipocyte triglyceride accumulation was attributed to total phenolic (F2) and chlorogenic acid enriched (F3-2) fractions that both inhibited by 75%. Pure compounds catechol (CAT) and chlorogenic acid (CA) also inhibited adipogenesis by 70%. Treatment with NBE, F1, F3-2, CAT and CA decreased p-AKT, whereas p-AMPK tended to increase with F1. The expression of SREBP1-c was not significantly modulated. In contrast, PPARγ decreased in all experimental groups that inhibited adipogenesis. CONCLUSIONS: These results demonstrate that fermented blueberry extract contains compounds with anti-adipogenic activity, which ca
n serve to standardize nutraceutical preparations from fermented blueberry juice and to develop novel compounds with anti-obesity properties.
From the journal
BMC complementary and alternative medicine
ISSN : 1472-6882

Bibliographic information

Language:
English
Type:
Journal Article
In AGRIS since:
2018
Volume:
17
Issue:
1
Extent:
24-24
Publisher:
BioMed Central
All titles:
"Fermented blueberry juice extract and its specific fractions have an anti-adipogenic effect in 3 T3-L1 cells"@eng
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Bibliographic information

Language:
English
Type:
Journal Article
In AGRIS since:
2018
Volume:
17
Issue:
1
Extent:
24-24
Publisher:
BioMed Central
All titles:
"Fermented blueberry juice extract and its specific fractions have an anti-adipogenic effect in 3 T3-L1 cells"@eng