AGRIS

Data provider:

Icon data provider

The National Agricultural Library is one of four national libraries of the United States, with locations in Beltsville, Maryland and Washington, D.C. It houses one of the world's largest and most accessible agricultural information collections and serves as the nexus for a national network of state land-grant and U.S. Department of Agriculture field libraries. In fiscal year 2011 (Oct 2010 through Sept 2011) NAL delivered more than 100 million direct customer service transactions.

Journal Article

Journal article

Complete germination of papaya (Carica papaya L. cv. `Maradol Roja´) somatic embryos using temporary immersion system type RITA® and phloroglucinol in semi-solid culture medium  [2017]

Posada-Pérez, Laisyn; Montesinos, Yenny, P.; Guerra, Diosdada, G.; Daniels, Dion; et al.

Access the full text

A critical factor in somatic embryogenesis protocols in papaya (Carica papaya L.) has been incomplete germination of somatic embryos due to formation of a basal callus, which prevents the emission of the radicle. This work aims to achieve complete germination of somatic embryos in liquid and semi-solid culture media. The effect of the culture conditions on germination of somatic embryos using the RITA® temporary immersion system were evaluated as well as the effect of phloroglucinol on germination of somatic embryos in semi-solid culture medium. The results of using the RITA® culture medium with a combination of 0.02 μM BAP and 2.90 μM gibberellic acid had a good response for total germination (100%) but somatic embryos had only partial germination with 400 mg fresh mass. However, the optimum inoculum density was 200 mg fresh mass of somatic embryos which produced 100% total germination and 95% somatic embryos with complete germination. Also, it was possible to achieve complete germination of somatic embryos with low callus formation (13%) using phloroglucinol at a concentration of 475.8 μM on semi-solid culture medium. This is the first report of two biotechnological strategies for complete germination of plants from somatic embryos in the papaya cultivar `Maradol Roja´.

Access the full text

Loading...

Bibliographic information

Language:
English
Type:
Journal Article
In AGRIS since:
2018
Volume:
53
Issue:
5
Extent:
505-513
Publisher:
Springer US
All titles:
" Complete germination of papaya (Carica papaya L. cv. `Maradol Roja´) somatic embryos using temporary immersion system type RITA® and phloroglucinol in semi-solid culture medium "

From the journal

In vitro cellular & developmental biology

ISSN : 1054-5476