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The National Agricultural Library is one of four national libraries of the United States, with locations in Beltsville, Maryland and Washington, D.C. It houses one of the world's largest and most accessible agricultural information collections and serves as the nexus for a national network of state land-grant and U.S. Department of Agriculture field libraries. In fiscal year 2011 (Oct 2010 through Sept 2011) NAL delivered more than 100 million direct customer service transactions.

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Paper

Written Paper

Fruit polyphenol oxidases: new data on an old problem  [1995]

Marques, L. (University of Montpellier II, Montpellier, France.); Fleuriet, A.; Macheix, J.J.;

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Many studies have been conducted on fruit polyphenoloxidases (PPO) and their involvement in browning but several unanswered questions still remain, e.g. enzyme latency, physical characteristics and structure-function relationships. Apple pulp PPO is associated with thylakoid membranes. Several forms were detected but the most important was a 64 kD monomer which, when folded by internal disulphide bonds, forms an active protein with an apparent molecular weight of 42 kD and optimal pH of 4.5-5 (inside thylakoid pH). This protein can be proteolysed into a 42 kD monomer that forms a 27 kD apparent molecular weight protein after folding. This latter protein is active over a broad pH range. Its resistance to proteolysis enabled development of an effective purification method. A highly specific polyclonal serum capable of detecting all proteolysed/nonproteolysed and folded/unfolded PPO forms was obtained. All of these different forms are present in apple pulp. The overall results are discussed in the light of recent reports

Bibliographic information

Language:
English
In AGRIS since:
1997
Publisher:
American Chemical Society
All titles:
"Fruit polyphenol oxidases: new data on an old problem"@eng
Other:
"references. Developed from a symposium sponsored by the Division of Agricultural Meeting of the Chemistry at the 208th National Meeting of the American Chemical Society, August 21-26, 1994, Washington, D.C"
"Enzymatic browning and its prevention.- Washington, DC (USA): American Chemical Society, 1995.- ISBN 08-412-32490 (alk. paper). p. 90-102"
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Bibliographic information

Language:
English
In AGRIS since:
1997
Publisher:
American Chemical Society
All titles:
"Fruit polyphenol oxidases: new data on an old problem"@eng
Other:
"references. Developed from a symposium sponsored by the Division of Agricultural Meeting of the Chemistry at the 208th National Meeting of the American Chemical Society, August 21-26, 1994, Washington, D.C"
"Enzymatic browning and its prevention.- Washington, DC (USA): American Chemical Society, 1995.- ISBN 08-412-32490 (alk. paper). p. 90-102"