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The National Agricultural Library is one of four national libraries of the United States, with locations in Beltsville, Maryland and Washington, D.C. It houses one of the world's largest and most accessible agricultural information collections and serves as the nexus for a national network of state land-grant and U.S. Department of Agriculture field libraries. In fiscal year 2011 (Oct 2010 through Sept 2011) NAL delivered more than 100 million direct customer service transactions.

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Journal Article

Journal Article

Reverse transcriptase activity in chicken embryo fibroblast culture supernantants is associated with particles containing endogenous avian retrovirus EAV-0 RNA  [1997]

Weissmahr, R.N. (University of Zurich, Zurich, Switzerland.); Schupbach, J.; Boni, J.;

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We have recently shown that live attenuated virus vaccines produced on chicken-derived cells contain low levels of particle-associated reverse transcriptase (RT). In both virus and corresponding control harvests produced on chicken embryo fibroblasts these activities were present at significantly higher concentrations than in the vaccines. In order to identify the putative retrovirus sequence responsible for this activity, a novel method for the selective PCR amplification of particle-associated retrovirus RNA that uses DNA primers complementary to the primer binding sites of the known exogenous retroviruses in combination with an anchor primer was applied. A product of the endogenous avian retrovirus family EAV-0. termed EAV-0B1, was reproducibly generated with a tRNA Trp-derived primer from the RT peak fraction of a sucrose density gradient run with a harvest of a live attenuated measles vaccine. In contrast, no products were detected with primers derived from tRNAPro, tRNALys(1,2) or tRNA3Lys. In the same fraction, genomic RNA of EAV-0 B1 was demonstrated by long PCR. Analysis of several sucrose density gradients from different harvests of various manufacturers demonstrated accumulation of, and colocalization with, RT activity for the EAV-0 B1 RNA but not for a chicken cellular mRNA. Synthesis of cDNA from EAV-0 B1 RNA was shown by endogenous RT reaction. Furthermore, complexes of naturally primed EAV-0 B1 RNA with RT were demonstrated. Taken together,
these data strongly suggest that EAV-0 is able to produce virus-like particles with an active RT
From the journal
Journal of virology (USA)
ISSN : 0022-538X

Bibliographic information

Language:
English
Type:
Journal Article
In AGRIS since:
1997
Volume:
71
Issue:
4
Start Page:
3005
End Page:
3012
All titles:
"Reverse transcriptase activity in chicken embryo fibroblast culture supernantants is associated with particles containing endogenous avian retrovirus EAV-0 RNA"@eng
Other:
"references"
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Bibliographic information

Language:
English
Type:
Journal Article
In AGRIS since:
1997
Volume:
71
Issue:
4
Start Page:
3005
End Page:
3012
All titles:
"Reverse transcriptase activity in chicken embryo fibroblast culture supernantants is associated with particles containing endogenous avian retrovirus EAV-0 RNA"@eng
Other:
"references"