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The Scientific Electronic Library Online - SciELO is an electronic library covering a selected collection of Brazilian scientific journals. The library is an integral part of a project being developed by FAPESP - Fundação de Amparo à Pesquisa do Estado de São Paulo, in partnership with BIREME - the Latin American and Caribbean Center on Health Sciences Information. Since 2002, the Project is also supported by CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico. The Project envisages the development of a common methodology for [...]

Journal Article

Journal article

The materno-fetal interface in llama (Lama guanicoe glama)  [2007]

Iturrizaga, David M.(Universidade de São Paulo Faculdade de Medicina Veterinária e Zootecnia Depto Cirurgia); Verechia, Flavia T.(Universidade Estadual Paulista Julio de Mesquita Filho); Santos, Tatiana C.(Universidade de São Paulo Faculdade de Medicina Veterinária e Zootecnia Depto Cirurgia); Bombonato, Pedro P.(Universidade de São Paulo Faculdade de Medicina Veterinária e Zootecnia Depto Cirurgia); et al.

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Samples from 9 llamas (28 through 36 weeks of gestation) were collected and fixed in 4% buffered paraformaldehyde (light microscopy) and in 2.5% buffered glutaraldehyde (transmission and scanning electron microscopy). The material was processed in paraplast and slides (5mm) were stained with HE, PAS, Masson-Trichrome, acid phosphatase and Perl's. The uteroferrin was immunolocalized. The results show that llama placenta is chorioallantoic, diffuse, folded and epitheliochorial, and the fetus is covered with an epidermal membrane. The trophoblast cells have variable morphology: cubic, rounded and triangular cells, with cytoplasm containing PAS-positive granules. Binucleated cells with large cytoplasm and rounded nuclei, as well as giant trophoblastic cells with multiple nuclei were also observed. Numerous blood vessels were observed beneath the cells of the uterine epithelium and around the chorionic subdivided branches. Glandular activity was shown by PAS, Perl's, and acid phosphatase positive reactions in the cytoplasm and glandular lumen, and by immunolocalization of the uteroferrin in the glandular epithelium. The uterine glands open in spaces formed by the areoles, which are filled by PAS-positive material. The llama fetus was covered by the epidermal membrane, composed of stratified epithelium, with up to seven layers of mono-, bi- or trinucleated cells. The high level of maternal and fetal vascularization surfaces indicates an intense exchange of subst
ances across both surfaces. The metabolic activity shown in the uterine glands suggests an adaptation of the gestation to the high altitudes of the natural habitat of this species.

From the journal

Pesquisa Veterinária Brasileira

ISSN : 0100-736X