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Journal Article

Journal article

Improved plant regeneration in Capsicum annuum L. from nodal segments  [2006]

Ahmad, N.; Siddique, I.; Anis, M.;

استعراض النص الكامل

Multiple shoots were induced by culturing nodal explants excised from 1-month-old aseptic seedlings of Capsicum annuum cv. Pusa Jwala on Murashige and Skoog (MS) medium supplemented with (0.1-10 microM) thidiazuron (TDZ). The rate of multiple shoot induction per explant was maximum (14.4+/-0.06) on MS medium supplemented with 1.0 microM TDZ. Regenerated shoots were elongated well on growth regulator free MS medium. Adventitious roots were induced two weeks after transfer of elongated shoots to MS medium supplemented with auxins (IAA, IBA or NAA) in different concentrations. Optimum root formation frequency was obtained in medium containing 1.0 microM IBA. Ex vitro rooting was also achieved by pulse treatment with 300 microM IBA for 10 min. Rooted shoots were transplanted in plastic pots containing garden soil (with 90% survival rate), where they grew well and attained maturity. Regenerated plants were phenotypically and cytologically normal.


Biologia Plantarum (Czech Republic)

الرقم التسلسلي المعياري الدولي (ردمد) : 0006-3134