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National Agricultural Research Centre (NARC), Islamabad  established in 1984, is the largest research centre of the Pakistan Agricultural Research Council (PARC). NARC, with a total land area of approximately 1400 acres, is located near Rawal Lake, six kilometers south-east of Islamabad. Physical facilities in term of experimental fields, laboratories, green houses, gene bank, library/ documentation, auditorium, machinery & lab equipment repair workshops, stores, hostels, cafeteria, audio visual studios, are also available at NARC.

Journal Article

Journal article

Involvement of Mycoplasma synoviae in respiratory distress cases of broilers  [2011]

Ehtisham-ul-Haque, S. (College of Veterinary and Animal Sciences, Jhang (Pakistan). Dept. of Microbiology); Rahman, S.U. (University of Agriculture, Faisalabad (Pakistan). Dept. of Microbiology); Siddique, M. (University of Agriculture, Faisalabad (Pakistan). Dept. of Microbiology); Qureshi, A.S. (University of Agriculture, Faisalabad (Pakistan). Dept. of Anatomy);

استعراض النص الكامل

Mycoplasma synoviae (MS) is an important pathogen of poultry worldwide, causing respiratory tract infection and infectious synovitis in chickens and turkeys. The study was designed to detect M. synoviae through serology, culture isolation and polymerase chain reaction (PCR) assay to document the involvement of MS infection in respiratory distress cases of broiler birds. The validated PCR assay amplifying the conserved gene region of 16SrRNA gene was applied for the detection of M. synoviae from culture as well as in clinical samples. The results indicated that 04 out of total 17 commercial broiler flocks showing respiratory distress signs were found positive with M. synoviae infection indicating 76.57% sero-positivity as, determined with rapid serum agglutination (RSA) test. Out of 85 clinical specimens (collected from sero-positive birds); M. synoviae culture isolation was successfully attained in 36 (42.35%) samples. Whereas, PCR test has detected 84 (98.82%) positive case


Pakistan Veterinary Journal (Pakistan)

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