Written Paper

Identification of contrasting genotypes for Fusarium wilt disease in Mucuna pruriens germplasm through combined in vitro screening and AFLP analysis  [2011]

Mahesh, S and Sathyanarayana, N*

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Fifteen germplasm accessions identified on earlier field data were screened for Fusarium wilt resistance using in vitro culturefiltrate approach. Infection and disease assessment were carried out based on wilt incidence and percentage mortality of theplants. Overall screening results revealed that 5 out of 15 accessions were moderate to highly resistant while 6 accessions werehighly susceptible to Fusarium wilt. The results were further supported by differences in root and shoot weight and lengthamongst susceptible and resistant genotypes. Polymorphism analysis based on AFLP data revealed only a sub-set of thesedivergent genotypes confirming to genetic divergence levels required for raising segregant population. Based on the results ofcombined analyses, the genotypes 500108KA and IC471870, 500101KA and 500155AP have been recommended as suitableparents for developing population for mapping Fusarium wilt resistance in Velvetbean.


Electronic Journal of Plant Breeding

ISSN : 0975-928X