Written Paper

Karyotype Analysis and Physical Mapping of rDNAs Using McFISH in Jeffersonia dubia Benth  [2005]

Kim, S.Y. (Chungnam National University, Daejeon, Republic of Korea) Kim, C.S. (Korea Forest Research Institute, Jeju, Republic of Korea) Choi, H.W. (Chungnam National University, Daejeon, Republic of Korea) Koo, D.H. (Chungnam National University, Daejeon, Republic of Korea) et al.

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Karyotype analysis and chromosomal locailization of 45S and 5S rDNAs using McFISH (multi-color fluorescence in situ hybridization) were carried out in Jeffersonia dubia Benth., which is one of medicinal plants belonging to Berberidaceae. The somatic metaphase chromosome number was 2n=2x=12 and the size of chromosomes ranged 1.95~3.50 ㎛. The chromosome complement consisted of two pairs of metacentrics (chromosomes 1 and 3), two pairs of submetacentrics (chromosomes 2 and 4) and two pairs of subtelocentrics (chromosomes 5 and 6). In McFISH, one pair of 45S rDNA site was detected on the centromeric region of chromosome 2 and three pairs of 5S rDNA sites were detected on the short arm of chromosome 4,5 and 6, respectively.


Korean Journal of Medicinal Crop Science

ISSN : 1225-9306