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The National Agricultural Library is one of four national libraries of the United States, with locations in Beltsville, Maryland and Washington, D.C. It houses one of the world's largest and most accessible agricultural information collections and serves as the nexus for a national network of state land-grant and U.S. Department of Agriculture field libraries. In fiscal year 2011 (Oct 2010 through Sept 2011) NAL delivered more than 100 million direct customer service transactions.

Journal Article

Journal article

Pilot-scale production and purification of the cellulolytic enzyme system from the white-rot fungus Phanerochaete chrysosporium  [1991]

Uzcategui, E. (University of Uppsala, Uppsala, Sweden); Raices, M.; Montesino, R.; Johansson, G.; et al.

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The fungus Phanerochaete chrysosporium (Sporotrichum pulverulentum) was grown on a pilot scale in a 100-liter automatic/fermenter and enzyme secretion was followed by continuous HPLC monitoring on an analytical Mono Q HR 5/5 column. The fermentation process yielded a culture filtrate with cellulose-degrading enzymes in a concentration several times higher than those reported from shaken-flask cultures. A quick and efficient downstream process involving ammonium sulfate precipitation afforded high recovery of the enzymes. A new purification approach for the cellulolytic enzyme system is also presented. Three 1,4-beta-D-glucan endoglucanases (EC and three 1,4-beta-D-glucan cellobiohydrolases (EC are brought to physicochemical homogeneity from the crude culture filtrate in a simple two- or three-step purification procedure. Ion-exchange, hydrophobic interaction, and gel chromatography are used as the main purification methods. Two of the purified enzymes have not been reported previously in the literature


Biotechnology and applied biochemistry (USA)

ISSN : 0885-4513