Written Paper

Chromosomal localization of rDNA in sunflower (Helianthus annuus L.) by fluorescent in situ hybridization  [1999]

Messina, R. (Udine Univ. (Italy). Dipartimento di Produzione Vegetale e Tecnologie Agrarie) Biagetti, M. (Universita della Tuscia, Viterbo (Italy). Dipartimento di Agrobiologia ed Agrochimica)

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The rDNA probes 5.8S-18S-25S and 5S of sunflower (Helianthus annuus L., 2n=34) have been physically mapped on chromosome spreads by fluorescent in situ hybridization. The biotin-labelled 5.8S-18S-25S rDNA probe showed a strong hybridization signal on three pairs of satellited chromosome at sites corresponding to the active NORs, and on a pair of fainter, not previously reported sites. The digoxigenin-labelled 5S rDNA probe was found to hybridize on two sites, one of which lies on a satellited chromosome