Written Paper

Distribution of chitinases and characterization of two chitinolytic enzymes from one-year-old Korean Ginseng (Panax ginseng C.A. Meyer) roots  [2010]

Moon, J.K., Ajou University, Suwon, Republic of Korea Han, B.K., Optifarm Solution Inc., Hanlim, Gunpo, Republic of Korea Kim, T.D.H., Ajou University, Suwon, Republic of Korea Jo, D.H., Ajou University, Suwon, Republic of Korea


We report the tissue-specific distribution of chitinolytic activity in Korean ginseng root and characterize two 31-kDa chitinolytic enzymes. These two enzymes (SBF1 and SBF2) were purified 70- and 81-fold with yields of 0.75 and 1.25%, respectively, and exhibited optimal pH and temperature ranges of 5.0-5.5 and 40-50℃. With [³H]-chitin as a substrate, K∧m and V∧max values of SBF1 were 4.6 mM and 220 mmol/mg-protein/h, respectively, while those of SBF2 were 7.14 mM and 287 mmol/mg-protein/h. The purified enzymes showed markedly less activity with p-nitrophenyl-N-acetylglucosaminide and fluorescent 4-methylumbelliferyl glycosides of D-N-acetylglucosamine oligomers than with [³H]-chitin. End-product inhibition of both enzymes demonstrated that both are endochitinases with different N-acetylglucosaminidase activity. Furthermore, the NH₂-terminal sequence of SBF1 showed a high degree of homology with other plant chitinases whereas the NH₂-terminal amino acid of SBF2 was blocked.


Biochemistry and Molecular Biology reports

ISSN : 1976-6696