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The University of Tasmania (UTAS) is a public research university primarily located in Tasmania, Australia. Founded in 1890

Journal Article

Journal article

The identification of a barley haze active protein that influences beer haze stability: Cloning and characterisation of the barley SE protein as a barley trypsin inhibitor of the chloroform/methanol type  [2007]

Robinson, LH; Juttner, J; Milligan, A; Lahnstein, J; et al.

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Previous pilot brewing trials have demonstrated that in the absence of a molecular weight (MW) 12,000 (barley silica eluate (SE) protein),the beer brewed from the malt of these SE ve varieties formed less haze after accelerated ageing than beers brewed using SE +ve maltvarieties. The previously described SE protein was characterised using comparative two-dimensional (2-D) gel electrophoresis immunoblotsof barley seed extracts from both SE +ve and SE ve varieties. The SE protein spot identified was excised and its partial sequencedetermined, after in-gel cleavage using trypsin and separation of the resulting fragments by reversed-phase high performance liquidchromatography (HPLC). N-terminal sequence analysis of the tryptic peptides from SE+ve and SE ve varieties identified the SE protein asthe barley trypsin inhibitor-CMe precursor (BTI-CMe). The mature BTI-CMe protein is 13.3 kDa and its functional gene is located onchromosome 3H. Cloning of the BTI-CMe protein demonstrated that both SE ve and SE +ve barley varieties contain a BTI-CMe proteinfamily member that is similar but has a consistently different sequence, primarily in the last 30 amino acid residues of their C-termini.