Data provider:

Icon data provider

Institutul de Cercetări şi Amenajări Silvice (ICAS) este o instituţie publică de interes naţional, cu personalitate juridică, aflată în coordonarea Ministerului Mediului şi Pădurilor (Legea nr.46/2008), specializată în cercetare ştiinţifică, dezvoltare tehnologică, proiectare de investiţii, acordarea de consultanţă tehnică de specialitate, precum şi pentru implementarea de tehnologii noi în vederea gestionării durabile a pădurilor.

Journal Article

Journal article

Micropropagation of an endangered species Pinus armandii var. Amamiana  [2008]

Katsuaki Ishii , Yoshihisa Hosoi, EmilioMaruyama, Sei-ichi Kanetan;

Access the full text

For micropropagation via organ culture, mature embryos were excised from the seeds of Pinus armandii. Franch. var. amamiana (Koidz.) Hatusima, an endangered species only inhabiting the south west islands of Japan. Adventitious buds were induced on the surface of the embryo on 1/2 DCR medium containing BAP, and they grew shoots after subculturing to medium containing activated charcoal or a low concentration of thidiazuron. From the elongated shoots, root primordia and roots were induced in medium containing IBA as an auxine. We found that a low concentration of zeatin or BAP added to the medium was beneficial for plant regeneration of mature embryos of this species. For micropropagation via somatic embryogenesis, embryogenic cell suspensions were induced from a mature and immature seed of P. armandii var. amamiana on MS liquid medium supplemented with 1 ¾M 2, 4-D and 3 ¾M BAP. The suspensions were incubated in the dark at 250. Induced suspension cells were transferred to ammonium free MS liquid medium supplemented with 1 ¾M 2, 4-D, 3 ¾M BAP and 30m M L-glutamine and subcultured every 2 weeks. In the other set of the experiment, the induction rate of somatic embryogenesis was high with ammonium free half strength MS medium. In order to develop somatic embryos, the suspension cells were transferred to ammonium free MS medium supplemented with 10 ¾M ABA, 0.2% activated charcoal, 10% PEG (MW6000), 30m M L-glutamine and 6% maltose. The cultures were incubated u
nder a 16h light/8h dark photoperiod. After 1-2 months of culture, differentiation of embryos progressed and cotyledonary embryos were obtained. These embryos were transferred on ammonium free MS solid medium under 16 h photoperiod. After 2-3 weeks plantlets with roots and green cotyledons were obtained. Plantlets were transplanted to vermiculite containing modified MS liquid medium in 200 ml culture flasks, then out planted after habituation procedure.

From the journal

Annals of Forest Research