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Founded in 1962, the CIHEAM is a Mediterranean intergovernmental organization devoted to the sustainable development of agriculture and fisheries, food and nutrition security and rural and coastal areas. It is composed of 13 member states (Albania, Algeria, Egypt, France, Greece, Italy, Lebanon, Malta, Morocco, Portugal, Spain, Tunisia and Turkey) and operates through its 4 Institutes based in Bari (Italy), Chania (Greece), Montpellier (France) and Zaragoza (Spain) and the Headquarters based in Paris.

The Old Yellow Enzyme (OYE) group of FMN-oxidoreductase studied showed significant effects in the regulation of programmed cell death in yeast and yet some of its physiological roles have remained a mystery. Lipid peroxidation is a physiological process where, under the influence of oxygen, lipid acids are degraded into smaller parts like alpha, beta- unsaturated aldehydes. These compounds ar ...

SUBJECT: cell death; genes; oxydoreductases; enzymes; cytoplasmatic organelles

Prolyl 4-hydroxylases (P4Hs) belong to the family of 2-oxoglutarate-dependent dioxygenases and catalyze the formation of 4-hydroxyproline, requiring 2-oxoglutarate and ? cofactor. Nine putative P4Hs have been identified up to now in the tomato genome. A reverse genetics approach, Virus-Induced Gene Silencing, was used to silence two P4Hs, P4H2 and P4H9. Transient silencing of P4H2 and P4H9 pro ...

SUBJECT: genetics; lycopersicon esculentum; genes; growth; plant viruses

Drainage of 40 (control), 60 and 80 percent drainage level was implemented in a closed hydroponics system (perlite bags) for cultivation of "Bianca" and "First Red" rose varieties under plastic greenhouse conditions for cut flower yield and quality. Control plants produced 84 and (63 mE-2) roses for "Bianca" and "First Red" respectively. Plants cultivated with highest drainage level (80 percen ...

SUBJECT: cut flower production; perlite; hydroponics; drainage; roses

The response of cucumber (Cucumis sativus L. var Crele) to salinity (2.2, 5 and 8 dS/m) using two different salinity sources (NaCl and CaCl2) and grown in a closed hydroponic system, using perlite as at substrate, was studied. Cucumber plants tolerated an EC value in the nutrient solution of 5 dS/m without reducing their total yield and plant yield and increasing the number of cucumbers produc ...

SUBJECT: salinity; yield losses; cucumus sativus; hydroponics; nutrient solutions

A new technique used in greenhouses for insect and fungi disease control is the use of UV-absorbing films. The benefits have already been proven, but the effect on the growth and development of tomato plants needs further investigations. The experiments were performed in three similar experimental plastic covered greenhouses, N-S oriented, located at the University of Thessaly near Volos, dur ...

SUBJECT: ultraviolet radiation; greenhouses; lycopersicon esculentum; hydroponics; growth

The behaviour of tomato plant (Sirine) and fruit quality factors, grown in a soilless culture system limestone) as a new substrate was examined in this study. The ceramic treatment was compared to the soil system, under the same greenhouse. For the soilless culture the plants were grown in two drained tanks of ceramic (bed) dogged 35 cm in the soil with a length of 30 meters and a width of 4 ...

SUBJECT: tomatoes; ceramics; lycopersicon esculentum; soilless culture; nutrient solutions

The existence of the evolutionary conserved role of prolyl 4-hydroxyalase (P4H) in oxygen sensing in many organisms might also suggest the involvement of the same enzyme in hypoxia signaling in plants. The P4H enzymes mediate a post translational modification and hydroxylation that lead to the degradation of the transcription factor HIF (hypoxia inducible factor). In the cells and tissues of m ...

SUBJECT: rna; genes; anoxia; enzymes; arabidopsis

The cellular mechanism by which oxygen tensions are sensed appears to be conserved among mammals, flies and worms. In response to low oxygen stress a transcription factor called HIF (hypoxia inducible factor) and a post-translational modification, hydroxylation, mediated by prolyl 4-hydroxylases (P4Hs) are the common characteristics of conditions of oxygen deprivation. P4H enzymes regulate HIF ...

SUBJECT: anoxia; arabidopsis; gene expression; pcr

Hydroxyproline-rich glycoproteins (HRGPs) are ubiquitous and relatively abundant in land plants and represent a structural component of cell walls. HRGPs are substrates for prolyl hydroxylation an are the focus of studies in relation to the functional significance of plant prolyl 4-hydroxylases. The aim of this research was the identification of HRGP cDNAs expressed in hypoxia-treated Dianthus ...

SUBJECT: molecular genetics; variety screening; mutants; cell walls; dianthus caryophyllus

Arabinogalactan proteins are cell wall structural proteins and are implicated in many aspects of plant development. Their proline residues are subjected to hydroxylation by prolyl 4-hydroxylases. We created hairpin RNAi constructs for silencing two putative tomato prolyl 4-hydroxylases for the development of molecular tools towards studying AGPs. Moreover, we investigated the significance of a ...

SUBJECT: tomatoes; protein content; hydroxylation; anoxia; gel electrophoresis