Seasonal changes of the different components of the inhibitor beta complex in potato tubers [beta inhibitor, abscisic acid, dormancy, rest]
1980
Franklin, J. | Hemberg, T. (Stockholm Univ. (Sweden). Botanical Inst.)
Seasonal changes of the inhibiting substances in the inhibtor Beta complex were studied in diethyl ether and methanol extracts of peelings from potato tubers, cv. Majestic, prepared during dormancy and cessation of dormancy. In both extracts the acidic ether soluble substances were investigated. From the methanol extracts also non-ether soluble but butanol soluble substances were studied. At each extraction time experiments were performed to determine the stage of rest in the tubers. Four different inhibitory zones were found with the Avena straight growth test of thin layer chromatograms. One of these was probably an artefact formed during chromatography. The level of all these substances decreased when rest ceased. - Thin layer chromatograms of the butanol fraction of methanol extracts, which should have contained the bound forms of the inhibitors, showed the same inhibitory zones as the ether fractions. This probably resulted from hydrolysis of the bound inhibitors when the extracts were chromatographed in an alkaline solvent system during purification
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