Direct radioimmunoassay of progesterone in milk using a radioiodinated progesterone derivative [in cows]
1980
Stupnicki, R. | Kula, E. (Institute of Animal Physiology and Nutrition, Jablonna (Poland))
A heterologous radioimmunoassay technique which allows the measurement of progesterone in whole milk, without extraction is described. An antiserum against progesterone-12 alphasuccinyl-BSA and progesterone-11 alpha-(2'-methyl)succinyl-('125)I-thyramine was used. When applied in pregnancy detection on day 20-22, progesterone levels of up to 6 ng/ml or above 8 ng/ml were indicative of an unsuccessful or a successful insemination, respectively. The sensitivity of the assay was 0.8 ng/ml, and the values found in cow milk ranged from 0.8 to 50 ng/ml.
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