Microvillus membrane vesicles from pig small intestine
1981
Christiansen, K. | Carslen, J. (Copenhagen Univ. (Denmark). Dept. of Biochemistry)
Microbvillus membrane vesicles from pig small intestine were isolated by a method based on hypotonic lysis, Mg('2+)-aggregation of contaminants and differential centrifugation. The purity of the membrane vesicles were established by measuring the activity of marker enzymes and the RNA and DNA content. The membranes were found free of contamination by other subcellular membrane fragments, except for a minor contamination with basolateral plasma membranes. The lipid composition was established and, based on weight percentage, the membrane contained neutral lipids, phospholipids, neutral glycolipids and gangliosides in the weight ratio of 18: 50: 29: 2 per cent. The amount of individual phospholipids and glycolipids were quantitated. Phosphatidylethanolamine, -choline, -serine, -inositol and sphingomyelin made up 17, 17, 6, 5 and 5 per cent, respectively of the total lipid. The major glycolipids were two monohezosylceramides containing glucose and galactose as the carbohydrate component, a dihexosylceramide containing galactose as the only carbohydrate component and two pentahezosylceramides cpntaining fucose, galactose, glucose and hexosamine (either N-acetylglucosamine or N-acetylgalactosamine) in the molar ratio of 1: 2: 1: 1.
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