Determination of 4-cholorindoleacetic acid methyl ester in Vicieae species by gas chromatography-mass-spectrometry [auxin, immature seeds, Vicia, Lathyrus, Lens, isotope dilution analysis, selected ion monitoring, leguminous seeds, Lathyrus sativus, Lathyrus maritimus, Lathyrus odoratus, Vicia sativa, Lens culinaris]
1981
Engvild, K.C. | Egsgaard, H. | Larsen, E. (Atomforsoegsanlaegget Risoe (Denmark). Landbrugsforsoegene)
The natural chlorinated auxin, 4-chloroindoleacetic acid methyl ester, was identified in immature seeds of Lathyrus sativus L., Lath of Lathyrus sativus L., Lathyrus maritimus (L.) Bigel and Lathyrus odoratus L. by thin layer chromatography and gas chromatography-mass spectrometry. In immature seeds of Vicia sativa L. and Lens culinaris Medik. the hormone was identified by selected ion monitoring. The hormone was determined quantitatively using pentadeuterated 4-chloroindoleacetic acid methyl ester as internal standard. Contents varied from 1 mg/kg fresh weight in Lathyrus sativus to 0.02 mg/kg in Lens culinaris. Lathyrus maritimus also contained indoleacetic acid methyl ester (0.3 mg/kg) besides the chlorinated analogue.
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