Some aspects of peroxidase synthesis by cultured peanut cells [Arachis hypogaea]
1981
Stephan, D. | Huystee, R.B. van (University of Western Ontario, London (Canada). Dept. of Plant Sciences)
Free and membrane-bound polysomes isolated from cultured peanut cells were used for in vitro protein synthesis. Using antibodies raised against a pure peroxidase isozyme, it was possible to show, by immunoprecipitation, that about twice as much peroxidase was synthesized on the membrane-bound polysomes as on the free polysomes. In vitro translation of poly(A)-containing messenger ribonucleic acid (mRNA) purified from total polysomal RNA, followed by immunoprecipitation with peroxidase specific antibodies indicated that about 2 per cent of the total poly(A)-containing mRNA coded for one major peroxidase isozyme in cultured peanut cells. In addition, two other peroxidase isozymes present in the cell suspension medium were purified by ion exchange chromatography. The peptide mappings derived from proteolysis of the three peroxidase fractions showed that there is a difference in the protein structure. Consequently, there may be three different loci for synthesis of peroxidase in these cultured cells.
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