High-performance liquid chromatography of fat-soluble vitamins
1982
Zonta, F. | Stancher, B. (Trieste Univ. (Italy). Ist. di Merceologia) | Bielawny, J.
Vitamins D(,2) and D(,3) and their corresponding previtamins and provitamins were resolved by reversed-phase high-performance liquid chromatography using a ternary solvent system (acetonitrile-methanol-water) pumped according to a gradient elution programme. The D vitamins were also resolved in the presence of other lipid-soluble vitamins (A, E and K(,1)) and carotene. The peaks were monitored with a UV-visible variable-wavelength detector and were detected at their maximum absorbance, resulting in maximum sensitivity. Lipid-soluble vitamins and carotene were resolved in extracts obtained from oils and butter, thus permitting their identification in a single chromatographic run.
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