Isolation of extracellular cytosine deaminase producing strain Arthrobacter sp. JH-13 and cultural conditions of its enzyme production

Jun, H.K.; Park, J.H. . Dept. of Microbiology

Isolation of extracellular cytosine deaminase producing strain Arthrobacter sp. JH-13 and cultural conditions of its enzyme production

1984

Jun, H.K. | Park, J.H. (Pusan National Univ. (Korea R.). Dept. of Microbiology)

An extracellular cytosine deaminase producing strain was isolated from soil samples. The enzyme obtained from the strain processed the substrate specificity to both cytosine and 5-fluorocytosine. The composition of optimum medium for the enzyme formation was 0.5% peptone, 0.5% meat extract, 0.5% soluble starch and 0.1% KCl. The optimum pH for the enzyme formation was 8.0%. When the microorganism was cultured aerobically in the above medium, enzyme production reached the maximum in 54 hrs. at 30 deg C. From the results of its morphological, cultural, physiological and biochemical properties, the strain was thought to be the genus Arthrobacter. It was therefore named Arthrobacter sp. JH-13

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