Hormonal effect and cytokinin autonomy in callus culture of Phaseolus vulgaris L.
1982
Kim, S.K. | Song, J.H. | Lee, K.W. (Seoul National Univ., Seoul (Korea R.). Dept. of Botany)
The effect of the activities of auxins and cytokinins in the growth of callus tissue derived from Phaseolus vulgaris L. was investigated. The synthetic auxin, picloram was the most effective in promoting callus growth and the range of effective concentrations (0.1micronM-32micronM) was broad. NAA promoted callus growth at relatively higher concentrations than other auxins tested. IAA was less effective in suporting callus growth. Cytokinin bearing saturated side chain(N6-isopentyladenine) was approximately 30 times more active than the corresponding unsaturated compound, n to the 6th power-(delta to the 2nd power-isopentenyl) adenine. Callus tissues previously grown on concentration lower and/or higher than optimal concentrations of cytokinins were better situated in the subsequent passage
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