Determination of bacterial number in a raw milk by ATP assay monitored by luciferin-luciferase bioluminescence reaction
1985
Han, S.H. | Kim, C.H. | Kim, J.B. | Shin, H.K. | Lee, S.B. (Konkuk Univ., Seoul (Korea R.). Coll. of Animal Husbandary)
Bacteriological quality of raw milk was determined by an ATP assay based on firefly bioluminescence reaction and was evaluated as a rapid method for the counting of bacteria in raw milk. The light yield was affected by the concentration of ATP, giving a straight line with a range of 10* (-10) to 10* (-6)M. When the number of bacteria in a raw milk counted by a conventional method was compared with an ATP assay, it gave a good correlation (r=0.74). This a new rapid method can be used for the determination of bacteriological quality of raw milk and applied to the other system, respectively
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